Volume 5, Issue 3 (Winter 2019)
SJNMP 2019, 5(3): 63-73 |
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1- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University , A.JAFARI_1392@YAHOO.COM
2- Department of Microbiology, Faculty of Medicine, Tabriz branch, Islamic Azad University
3- Department of Microbiology, Ahar Branch, Islamic Azad University
4- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University
2- Department of Microbiology, Faculty of Medicine, Tabriz branch, Islamic Azad University
3- Department of Microbiology, Ahar Branch, Islamic Azad University
4- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University
Abstract: (2617 Views)
Background & Aim: The incidence of drug resistance against chemical antimicrobial drugs, has led in recent years to the use of medicinal plants in the treatment of infections is high. The aim of this study was to determine the antibacterial effect of plants Centaurea depressa M.B., Reseda lutea and Fumaria asepala is on some pathogenic bacteria.
Materials & Methods: plants of the city of Marand East Azerbaijan province of Iran in the spring were collected by botanists and Agriculture Organization, C.depressa M.B., R.lutea and F.asepala and medicinal plants were approved. After preparation of the plant extracts, concentration concentrations 50- 400 mg / ml, the extract from the agar well diffusion and disk diffusion method on bacteria were studied. The minimum bactericidal concentration and the minimum inhibitory concentration (MIC / MBC) were performed by dilution test.
Results: The results showed that the inhibitory effect of alcoholic extracts of C.depressa MB and R.lutea the Gram-positive bacteria, Gram-negative bacteria is more than alcoholic extract of F.asepala The inhibitory effect on gram-negative bacteria than bacteria Gram positive. Analysis of test MBC / MIC showed that extracts of medicinal plants most susceptible to B.cereus and E. coli was the least sensitive.
Materials & Methods: plants of the city of Marand East Azerbaijan province of Iran in the spring were collected by botanists and Agriculture Organization, C.depressa M.B., R.lutea and F.asepala and medicinal plants were approved. After preparation of the plant extracts, concentration concentrations 50- 400 mg / ml, the extract from the agar well diffusion and disk diffusion method on bacteria were studied. The minimum bactericidal concentration and the minimum inhibitory concentration (MIC / MBC) were performed by dilution test.
Results: The results showed that the inhibitory effect of alcoholic extracts of C.depressa MB and R.lutea the Gram-positive bacteria, Gram-negative bacteria is more than alcoholic extract of F.asepala The inhibitory effect on gram-negative bacteria than bacteria Gram positive. Analysis of test MBC / MIC showed that extracts of medicinal plants most susceptible to B.cereus and E. coli was the least sensitive.
Conclusion: the plant extracts tested in vitro on the growth of pathogenic bacteria has significant inhibitory effects. In order to further clinical trials are necessary clinical application of these extracts.
Type of Study: Research |
Subject:
General
Received: 2019/08/21 | Revised: 2019/12/30 | Accepted: 2019/12/30 | Published: 2019/12/22 | ePublished: 2019/12/22
Received: 2019/08/21 | Revised: 2019/12/30 | Accepted: 2019/12/30 | Published: 2019/12/22 | ePublished: 2019/12/22
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