Volume 5, Issue 1 (Summer 2019)                   SJNMP 2019, 5(1): 61-68 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jafari-sales A, Shadi-Dizaji A. Evaluation of Inhibitory Effect of Methanol Extract of Allium Sativum in vitro on Staphylococcus aureus and Escherichia coli. SJNMP. 2019; 5 (1) :61-68
URL: http://sjnmp.muk.ac.ir/article-1-229-en.html
1- Department of Microbiology School of Basic Sciences, Kazerun Branch, Islamic Azad University , a.jafari_1392@yahoo.com
2- Department of Biotechnology, Ataturk University, Turkey.
Abstract:   (511 Views)
Background & Aim: Today, increased use of antibiotics to treat infections has led to increased resistance to antibiotics in bacteria strains. Therefore, extensive efforts have been dedicated to finding new compounds as a suitable replacement for antibiotics. This study aimed to compare and evaluate the antibacterial effects of Allium Sativum methanol extract on the growth of several pathogenic bacteria.
Materials & Methods: In this empirical-laboratory research, the methanol extract of the plant was prepared after collecting and conducting pharmacognosy studies. Afterwards, the antibiotic effects of the plant were assessed at 20-400 mg/ml concentrations using the well diffusion method. Moreover, the minimum inhibitory concentration (MIC) was determined applying the tubular dilution method.
Results: In this research, the methanol extract of Allium Sativum prevented the growth of Staphylococcus aureus and Escherichia coli. At 6.25 mg/ml concentration, the methanol extract of Allium Sativum had the highest inhibitory effect on the growth of Staphylococcus aureus. Meanwhile, the concentration of 12.5 mg/ml had the most bactericidal effect. Conclusion: According to the results of the study, Allium Sativum might be recognized useful when used to treat bacterial infections independently after completing additional research. Therefore, this compound might be applied as a substitute for conventional chemical drugs to treat infections.
Full-Text [PDF 549 kb]   (203 Downloads)    
Type of Study: Research | Subject: Special
Received: 2019/02/26 | Revised: 2020/01/18 | Accepted: 2019/04/14 | Published: 2019/06/9 | ePublished: 2019/06/9

1. Digrak M, Alma MH, Ilçim A. Antibacterial and antifungal activities of Turkish medicinal plants. Pharmaceutical Biology. 2001;39(5):346-350. [DOI:10.1076/phbi.39.5.346.5903]
2. Skaltsa HD, Lazari DM, Chinou IB, Loukis AE. Composition and antibacterial activity of the essential oils of Stachys candida and S.chrysantha from southern Greece. Planta Medica. 1999;65(03):255-256. [DOI:10.1055/s-2006-960471]
3. Jafari-Sales A, Jafari B, Sayyahi J, Zohoori-Bonab T. Evaluation of antibacterial activity of ethanolic extract of malva neglecta and althaea officinalis l. On antibiotic-resistant strains of staphylococcus aureus. J Biol Today World. 2015;4(2):58-62. [DOI:10.15412/J.JBTW.01040205]
4. Jafari-Sales A, Shahniani A, Fathi R, Malekzadeh P, Mobaiyen H, Bonab FR. Evaluation of Antibacterial Activity of Essential Oil of Ziziphora clinopodioides and Achillea wilhelmsii on Antibiotic-resistant Strains of Staphylococcus aureus. IMMINV. 2017; 2(2):49-56. [DOI:10.24200/imminv.v2i2.58]
5. Mobaiyen H, Jafari Sales A, Sayyahi J. Evaluating antimicrobial effects of centaurea plant's essential oil on pathogenic bacteria: staphylococcus aureus, staphylococcus epidermidis, and escherichia coli isolated from clinical specimens. J Fasa Univ Med Sci. 2016. 5(4): p. 479-487.
6. Sales AJ, Evaluation of antibacterial activity of ethanol extract of Lavandula Stoechas L. plant on antibiotic-resistant strains Of Staphylococcus Aureus. J Curr Res Sci. 2014. 2(6):641.
7. Sales AJ, Bagherizadeh Y, Malekzadeh P, Ahmadi B, Bonab FR. Evaluation of the Antimicrobial Effects of Essential Oil of Reseda Lutea L. on Pathogenic Bacteria: Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli. Arch Clin Microbiol, 2017;8(3): 1-6. [DOI:10.4172/1989-8436.100041]
8. Sales AJ, Shadbad NN, Kaleybar VP. The Investigation of the Antibacterial effects of Ethanol extract of Cichorium intybus L. on Antibiotic-resistant Staphylococcus aureus strains. Bull. Env. Pharmacol. Life Sci. 2015; 4(4):161-164.
9. Qi Z. WHO Traditional Medicine Strategy. 2014-2023. Geneva: World Health Organization, 2013.
10. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev, 1999; 12(4):564-582. [DOI:10.1128/CMR.12.4.564]
11. Salama AA, et al. Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites. J Parasitol Res. 2014; 113(1):275-283. [DOI:10.1007/s00436-013-3654-2]
12. Wolde T, Kuma H, Trueha K, Yabeker A. Anti-Bacterial Activity of Garlic Extract against Human Pathogenic Bacteria. J Pharmacovigil. 2018; 6(253):2-8.
13. Bakri IM, Douglas CWI. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol. 2005;50(7):645-651. [DOI:10.1016/j.archoralbio.2004.12.002]
14. Jazani NH, Shahabi S, Ali AA, Zarrin S, Daie NA. In vitro antibacterial activity of garlic against isolates of Acinetobacter sp. J Biol Sci. 2007;7(5):819-822. [DOI:10.3923/jbs.2007.819.822]
15. Sivam GP. Protection against Helicobacter pylori a nd Other Bacterial Infections by Garlic. Journal of Nutrition. 2001;131(3):1106S-1108S. [DOI:10.1093/jn/131.3.1106S]
16. Benavides GA, et al. Hydrogen sulfide mediates the vasoactivity of garlic. Proc Natl Acad Sci. 2007;104(46):17977-17982. [DOI:10.1073/pnas.0705710104]
17. Karuppiah P, Rajaram S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pac J Trop Biomed. 2012;2(8):597-601. [DOI:10.1016/S2221-1691(12)60104-X]
18. Onyeagba RA, Ugbogu OC, Okeke CU, Iroakasi O. Studies on the antimicrobial effects of garlic (Allium sativum Linn), ginger (Zingiber officinale Roscoe) and lime (Citrus aurantifolia Linn). Afr J Biotechnol. 2004; 3(10):552-554. [DOI:10.5897/AJB2004.000-2108]
19. Jafari-Sales A, Bolouri P. Evaluation of the antimicrobial effects of Glycyrrhiza glabra l. on some gram positive and gram negative pathogenic bacteria in laboratory conditions. Jorjani Biomed J. 2018;6(4):78-84. [DOI:10.29252/jorjanibiomedj.6.4.78]
20. Alorainy MS. Evaluation of antimicrobial activity of garlic (Allium sativum) against E. coli O157: H7. J Agr Vet Sci. 2011;4:149-157.
21. Belguith H, Kthiri F, Chati A, Sofah AA, Hamida JB, Landoulsi A. Study of the effect of aqueous garlic extract (Allium sativum) on some Salmonella serovars isolates. Emir. J. Food Agric, 2010;22(3): 189-93. [DOI:10.9755/ejfa.v22i3.4889]
22. Zhang, X. Three supplementary methods for analyzing cytotoxicity of Escherichia coli O157: H7. J Microbiol Meth. 2016;120:34-40. [DOI:10.1016/j.mimet.2015.11.011]
23. Alzowahi FA, Abu-Taleb A, As-Suhbani A, Kadam TA. The inhibitory effects of garlic extract and its fractions against some Enterobacteriaceae spp. isolated from sprouted Mung bean. Int J Curr Microbiol App Sci. 2013;2(7):104-115.
24. Daka D, Awole M, Assessment of the antibacterial effect of crude preparation of garlic (Allium sativum) on diarrhea causing bacteria: an In vitro, study. Asian J Med Sci. 2009;1(1):12-14.
25. Gull I, Saeed M, Shaukat H, Aslam SM, Samra ZQ, Athar AM. Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Ann Clin Microb Anti. 2012;11(1):8. [DOI:10.1186/1476-0711-11-8]
26. Iwalokun BA, Ogunledun A, Ogbolu, DO, Bamiro SB, Jimi-Omojola J. In vitro antimicrobial properties of aqueous garlic extract against multidrug-resistant bacteria and Candida species from Nigeria. J Med Food. 2004; 7(3):327-333. [DOI:10.1089/jmf.2004.7.327]
27. Sabahi M, esmaeili R, dastan D, Alikhani M Y. Antibacterial Activity of Aqueous and Alcoholic Extracts of Garlic and Aloe Vera Against Clinical Isolates of Staphylococcus aureus, Pseudomonas aeruginosa and E.coli . Iran J Med Microbiol. 2018;12(4):288-293. [DOI:10.30699/ijmm.12.4.288]
28. Jabar MA, Al-Mossawi A. Susceptibility of some multiple resistant bacteria to garlic extract. Afr J Biotechnol. 2007;6(6):771-776.
29. Majnooni MB, Abiri R, Afnanzade NS, Malek Khatabi P. Study of antibacterial effects of hydro-alcoholic extract of 8 medicinal herbs against vancomycin resistant staphylococcus aureus. JMP. 2012;1(41):103-110.
30. Sadeghian A, Ghazvini K, Antimicrobial activity of garlic extract against Shigella. IJBMS. 2015;27(3):142-144.

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | Scientific Journal of Nursing, Midwifery and Paramedical Faculty

Designed & Developed by : Yektaweb